HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY ASSAY FOR EPOXIDE HYDROLASE ACTIVITY AND THE DETERMINATION OF PHENOXYPROPANE-1 ,ZDIOLS E. VAN DEN EECKHOUT* and P. DE MOERLOOSE
نویسنده
چکیده
A simple, rapid and sensitive high-performance thin-layer chromatographic assay for the determination of epoxide hydrolase activity in rat liver homogenates is described. It is extended to the determination of a series of phenoxypropane-1,Z diols. The hydrolase assay has the advantages of using a readily available substrate, 2,3-epoxypropyl4-methoxyphenyl ether, of multiple sample application, and of the simultaneous determination of reaction product (diol) as well as unchanged substrate (epoxide). The use of an internal standard, Cnitroanisole, results in high sensitivity and good reproducibility of the proposed method. The limit of diol detection is 20 pmol.
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